Figure 7.

TLR2 exerts an inhibitory effect on Th1 and Tc1 responses against YF-17D. Wild-type or TLR2^−/− mice were vaccinated subcutaneously with YF-17D. Control mice were injected with supernatants from mock transfected SW-480 cells. 5 or 10 d later, the draining lymph nodes were harvested and single cell suspensions cultured in vitro in media alone, or with a combination of CD8⁺ T and CD4⁺ T cell epitopes from YF-17D. 3 d later, the induction of IFN-γ was measured by intracellular cytokine staining (A and B). These data are representative of four independent experiments with three mice per group in each experiment.