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. 2006 Feb 20;203(2):437–447. doi: 10.1084/jem.20051775

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Copyright © 2006, The Rockefeller University Press

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Figure 6. — IFN-γ production of OT-I T cells after restimulation with cognate antigen. (A) Liver transplant recipients with restricted intrahepatic or systemic antigen presentation were killed on day 4 after initial antigen injection. Cell isolates from livers, spleens, and lymph nodes were restimulated with SIINFEKL peptide for 6 h and their IFN-γ production was assessed by intracellular cytokine staining. (B) Average percentage of IFN-γ producers among isolated OT-I T cells. These experiments show that priming in liver produces fully functional CD8⁺ T cells. Data are representative of three independent experiments with three mice per group, and the bars in B represent the mean ± SEM.