Figure 4.

Binding of KLRG1 to three members of the classical cadherin family. (A) E-cadherin expression on mouse cell lines that bound the KLRG1 tetramer. The indicated cell lines were stained with a mAb to mouse E-cadherin (ECCD-2) and FITC-anti–mouse IgG (filled histograms) or FITC-anti–mouse IgG alone (solid lines). (B and C) BW5147 cells were retrovirally transduced with vectors for bicistronic expression of EGFP and the mouse cadherins indicated. The cells were stained with the KLRG1 tetramer (B) or tested for stimulation of the mouse KLRG1 reporter cells (C). In C, representative data (mean ± SD of triplicate samples) from one of three independent experiments are shown.