Figure 6.

H2-M3 deficiency has no effect on secondary anti-listerial response. 1 mo after primary LM infection, M3^−/− (N6) and WT mice were rechallenged with 10⁵ CFU of LM. (A) The bacterial burdens in the livers were determined on days 2 and 3 after secondary LM infection. (B) The percentage of CD8⁺ T cells in the spleens of LM-infected mice on the indicated days was determined by flow cytometric analysis. Data shown are mean ± SEM of five to six mice per group per time point. (C) Comparable LM-specific cytolytic activity in M3^−/− and WT mice after secondary LM infection. 3 d after LM reinfection, splenocytes from indicated mice were activated with Con A and examined for CTL activity to ⁵¹Cr-labeld LM-infected J774-K^b target cells. Data are representative of results obtained from three mice per group