Table II.
Number of mAbs generated by immunization with irradiated sporozoites or αDEC-CS plus maturation stimulus
| Immunization method | Number of screened clonesa | Number of positive clones (% of the total screened) |
Positive clones (%)b | |
|---|---|---|---|---|
| Major repeat | Minor repeat | |||
| Irradiated sporozoitesc | 1,277 | 10 (0.8) | 100 | 0 |
| αDEC-CS+anti-CD40+polyI:Cd | 736 | 121 (16.4) | 55.4 | 44.6 |
a
Growing clones were screened for antibody secretion.
b
Growing clones were screened for reactivity against sequences representing the major repeat (QGPGAP)2 or the minor repeat (QQPP)2 of CSP.
c
Prime and boost were performed i.v. with 7.5 × 104 irradiated sporozoites in the interval of a month. The fusion was performed 3 d after the boost dose.
d
Prime i.p. with 10 μg αDEC-CS plus anti-CD40 plus poly I:C. 21 wk later, the boost was performed i.p. with 10 μg αDEC-CS. The fusion was performed 3 d after the boost dose.