Table III.
mAb subclasses generated by the immunization with irradiated sporozoites or with αDEC-CS plus maturation stimulus
| Immunization methoda | Positive clones against the major repeat (%)b | Positive clones against the minor repeat (%)b | ||||||
|---|---|---|---|---|---|---|---|---|
| IgG1 | IgG2a | IgG2b | IgG3 | IgG1 | IgG2a | IgG2b | IgG3 | |
| Irradiated sporozoites | 70 | 10 | 0 | 20 | 0 | 0 | 0 | 0 |
| αDEC-CS+anti-CD40+poly I:C | 56.7 | 9 | 7.5 | 26.9 | 25.9 | 25.9 | 40.7 | 7.4 |
a
Mice were immunized as described in Table II.
b
All positive clones were rescreened against the major and minor repeats of CSP using anti–mouse subclass-specific secondary antibodies.