Figure 5.

Long-lived gag p24-memory to DC-targeted vaccine. BALB/c mice were immunized i.m. with two doses of 50 μg HIV gag p24 DNA or i.p. with 5 μg anti–DEC-p24 and control Ig-p24 mAb with maturation stimulus. C57BL/6 mice were similarly immunized, but the fusion antibodies and maturation stimulus were given s.c. CD4⁺ T cells were enriched by depleting MHC II⁺ and CD8⁺ cells from the lymph node (30 wk after BALB/c immunization) or spleen (at 19 wk for C57BL/6), CFSE labeled, and restimulated with CD11c⁺ DCs plus the peptide pools as indicated, anti-CD3 and anti-CD28, or medium. Proliferation was evaluated by CFSE dilution at 4 d. Numbers are the percentage of CD4⁺ proliferating T cells. Control PBS-immunized mice showed similar proliferative responses to control Ig-p24 mAb or two doses of DNA gag p24 vaccine. Data are representative of two to three experiments on the same batch of immunized mice.