Table II.

HIV gag p24 mimetope peptides identified from 15-mer peptide pools to stimulate IFN-γ secretion by CD4⁺ T cells in three MHC haplotypes after anti–DEC-p24 immunization

Strain	HIV gag p24 Peptide pool	Reactive peptide
C3H	2	AAEWDRLHPVHAGPI (aa 209–223)
	4	YSPTSILDIRQGPKE (aa 277–291)
BALB/c	1	SPEVIPMFSALSEGA (aa 165–179)
	3	PVGEIYKRWIILGLN (aa 257–271)
C57BL/6	1	QAISPRTLNAWVKVV (aa 145–159)
	4	VDRFYKTLRAEQASQ (aa 297–311)

5 μg anti–DEC-p24 antibody in combination with 25 μg anti-CD40 and 50 μg poly IC was administered either i.p. to BALB/c mice or s.c. to C57BL/6 or C3H mice as in Fig. 4 A. After 17 d, splenic CD4⁺ T cells were restimulated with CD11c⁺ DCs and single peptides from the different reactive HIV gag p24 peptide pools (2 μg/ml) for 2 d. IFN-γ secretion was evaluated by ELISPOT.