Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Mar 20;203(3):553–561. doi: 10.1084/jem.20052438

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — The TLR9 pathway was required for generation of IgG2a and 2b anti-DNA/polyreactive autoantibodies. IgG subclass analysis of autoantibodies in the serum of 7–11-wk-old 56R⁺RIIB^−/−TLR9^+/+.B6 (n = 7), 56R⁺RIIB^−/−TLR9^+/−.B6 (n = 19), 56R⁺RIIB^−/−TLR9^−/−.B6 (n = 7), or 56R⁺RIIB^−/−MyD88^−/−.B6 (n = 6) mice as determined by ANA (A), anti-DNA (B), antiglomerular basement membrane (anti-GBM) (C), and anticardiolipin (D) ELISA, indicated a reduction of the autoreactive IgG2a_a and IgG2b subclasses for both genotypes (P = 0.007 and P = 0.03, respectively, for the ANA ELISA). (E) Polyreactivity was analyzed by preabsorbing the samples on the indicated ELISA plates; G: GBM; C: cardiolipin; G,C: GBM + cardiolipin. (F) TLR9-deficient IgM_a ⁺ naive splenic B cells retained the intact 56R VDJ4_H transgene, whereas IgG2a and 2b positive TLR9-deficient GC and splenic plasma cells did not use the intact 56R VDJ4_H transgene (compare with Fig. 3).