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. 2006 Mar 20;203(3):573–582. doi: 10.1084/jem.20051520

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Copyright © 2006, The Rockefeller University Press

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Figure 3. — p27^Kip1 causes G1 arrest without effect on survival in D1 cells. D1 cells were transfected with p27^Kip1 and cultured with IL-7 for certain time points. Cell cycle and survival were analyzed by PI staining after flow cytometric analysis. G1 peak, S phase, G2/M phase, and apoptotic populations are indicated. Ap, apoptosis. Percentage of cells in different populations was calculated by ModFit LT software. The results are representative of three independent experiments.