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. 2006 Mar 20;203(3):573–582. doi: 10.1084/jem.20051520

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Copyright © 2006, The Rockefeller University Press

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Figure 4. — siRNA knockdown of p27^Kip1 expression protects from cell cycle arrest induced by IL-7 deprivation in vitro. D1 cells transfected with either pMIG-hU6-sip27^Kip1 (sip27^Kip1, small interfering p27^Kip1) or pMIG-hU6-NC (Control, scrambled siRNA control) were kept in IL-7 or without IL-7 for 15 h. Total cell extracts were subjected to immunoblotting with antibodies against p27^Kip1 and β-actin (top). Cell cycles were determined by PI staining (bottom). Percentage of cells in different populations was calculated by ModFit LT software. A representative experiment of three performed is shown.