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. 2006 Mar 20;203(3):573–582. doi: 10.1084/jem.20051520

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Copyright © 2006, The Rockefeller University Press

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Figure 7. — PKCθ is activated and inhibition of PKCθ prevents cell cycle G1 arrest after IL-7 withdrawal. (A) PKC activation was analyzed in D1 cells of IL-7 withdrawal for 12 h. Cell lysates were resolved by SDS-PAGE and immunoblotted using a phospho-PKC sample kit. Total PKCθ was measured as a loading control. (B) PKCθ activation was determined in D1 cells after IL-7 deprivation. Cell lysates were made from cells deprived of IL-7 for 4, 8, and 12 h, and PKCθ activation was assayed by immunoblotting using an antibody specific for PKCθ phosphorylation at threonine 538. (C) D1 cells were cultured without IL-7 and treated with 5 μM Gö6850 for 15 h. Cell cycle was analyzed by PI staining and the percentage of cells in different populations was calculated by ModFit LT software. Results were pooled from three experiments.