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. 2006 Apr 17;203(4):941–951. doi: 10.1084/jem.20052124

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Copyright © 2006, The Rockefeller University Press

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Figure 5. — Supernatants from LS-174T cells treated with PGE₂ induce endothelial migration and tube formation. (A) Cell movement was evaluated by counting the number of cells that migrated toward conditioned medium in the undersurface of the filter. The data were represented as the mean ± SE of cell numbers of three independent experiments performed in triplicate. (B and C) The Py-4-1 cells (top) and BPMVECs (bottom) form capillary-like structures on growth factor–reduced Matrigel in vehicle- or 1 μM PGE₂-treated conditioned media (B). The number of the intersections between branches of assembled endothelial cell networks was counted in whole field. Data were represented as the mean ± SE of intersection numbers of three independent experiments (C). Asterisks represent statistical differences (P < 0.05; Student's t test). (D) Quantitative real-time PCR analysis of the levels of CXCR2 mRNA in LS-174T and Py-4-1 cell lines was performed.