Figure 6.

CXCL1 mediates the effects of PGE₂ on endothelial migration and tube formation. (A–C) The effects of CXCL1 neutralizing antibody on endothelial migration. The Py-4-1 cells (A and B) or BPMVCs (C) were cultured and treated in the same conditions as noted in Fig. 4 A. The supernatants from LS-174T cells (A or C) or HCA-7 cells (B) were pretreated with either 5 μg of normal mouse IgG or anti-hCXCL1 mouse monoclonal antibody for 1 h before being placed in the lower chamber. The data were represented as the mean ± SE of cell numbers of three independent experiments performed in triplicate. (D) Recombinant CXCL1 induces endothelial cell migration. The Py-4-1 cells and BPMVCs were cultured and treated in the same conditions as noted in Fig. 4 A. The serum-free DMEM medium with indicated amounts of CXCL1 was placed in the lower chamber. The data were represented as the mean ± SE of cell numbers of three independent experiments performed in triplicate. (E–G) The effects of CXCL1 neutralizing antibody on endothelial tube formation. The Py-4-1 cells (E and F) and BPMVCs (G) were cultured and treated in the same conditions as noted in Fig. 4 B. The supernatants from LS-174T (E and G) or HCA-7 (F) cells were pretreated with either 5 μg of normal mouse IgG or anti-hGROα mouse monoclonal antibody for 1 h before being placed in the lower chamber. Data were represented as the mean ± SE of intersection numbers of three independent experiments.