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. 2006 Apr 17;203(4):1007–1019. doi: 10.1084/jem.20051342

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Copyright © 2006, The Rockefeller University Press

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Figure 2. — β-dystroglycan loss in inflamed vessels. Immunofluorescence for β-dystroglycan and pan-laminin reveals continuous β-dystroglycan staining bordering the parenchymal BM in noninflamed vessels (A–C) and loss of β-dystroglycan in inflamed vessels only (arrows in D and E). Triple staining for pan-laminin with β-dystroglycan and CD45 (G–I), or agrin and CD45 (J and K) reveals loss of β-dystroglycan at sites of leukocyte infiltration (G and I), despite continuous pan-laminin (H) and agrin (J and K) staining. Double β-dystroglycan and GFAP staining indicates presence of astrocyte endfeet surrounding inflamed vessels (arrows in F). Perlecan staining of the parenchymal BM is continuous around perivascular cuffs, with higher intensity staining in endothelial BMs (L). Western blot reveals ∼200 kD agrin core protein and the characteristic broad mol wt smear in stage 4 EAE and noninflamed brains (Cont.) and purified agrin (M). The table summarizes immunofluorescence and Western blot data. Images are from different specimens and represent results from 10 mice. Bars, 40 μm.