Figure 3.

IL-25 induces NBNT, c-kit⁺, FcɛR1⁻ cell population in the MLN. (A) NBNT, c-kit⁺, FcɛR1⁻ cells are deficient in the MLN of il25 ^−/− animals 5 d after infection with N. brasiliensis (Nb). Cohorts of wild-type and il25 ^−/−-infected mice (n = 5–6) were killed and the NBNT, c-kit⁺, FcɛR1⁻ cell population assessed by flow cytometry. (B) BALB/c mice (n = 3–5) were administered with rIL-25 and flow cytometry was used to analyze NBNT, c-kit⁺, FcɛR1⁻ cells in the MLN and spleen 4 d later. (C) Cohorts of wild-type and rag1 ^−/− mice (n = 3–5) were administered with rIL-25 and flow cytometry used to analyze NBNT, c-kit⁺, FcɛR1⁻ cells in the MLN 4 d later. (D) Cohorts of wild-type and il4 ^−/− il5 ^−/− il9 ^−/− il13 ^−/− mice (n = 3–5) were administered with rIL-25 and flow cytometry used to analyze NBNT, c-kit⁺, FcɛR1⁻ cells in the MLN 4 d later. Data are representative of two to three experiments.