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. 2006 Apr 17;203(4):1105–1116. doi: 10.1084/jem.20051615

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Copyright © 2006, The Rockefeller University Press

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Figure 5. — Analysis of purified NBNT, c-kit⁺, FcɛR1⁻ cells from IL-25–treated wild-type mice. (A) NBNT, c-kit⁺, FcɛR1⁻ cells were isolated by cell sorting and cytospin preparations of sorted cells stained with Giemsa or Toludine. (B) Flow cytometry of cell surface marker expression. (C) Analysis of gene expression. neg, no template control; mast, bone marrow–derived mast cells; c-kit⁺, NBNT, c-kit⁺, FcɛR1⁻ cells; Th2, splenocytes stimulated under Th2 cell differentiation conditions for 48 h; pos, image clone used as positive control; CCR, chemokine receptor; MCP, mast cell protease; EPO, eosinophil peroxidase. EPO positive control was IMAGE clone 30044595. CCR1 positive control was IMAGE clone 3992755.