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. 2006 Apr 17;203(4):871–881. doi: 10.1084/jem.20050930

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Copyright © 2006, The Rockefeller University Press

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Figure 2. — Tumor-associated macrophages express B7-H4. (a and b) Tumor ascites macrophages express B7-H4. FACS analysis showed that ascites macrophages, but not control nonmalignant ascites macrophages, or fresh normal blood monocytes expressed B7-H4. Results are expressed as the mean of the percentage of B7-H4⁺ cells ± SEM in total macrophages. Filled histogram, B7-H4 staining; open histogram, isotype control. (c) High prevalence of B7-H4⁺ tumor macrophages in tumor ascites. FACS analysis was used to determine the prevalence of B7-H4⁺ tumor macrophages and CD3⁺CD4⁺CD25⁺ cells in tumor ascites (*, P < 0.001). Results are expressed as the mean of percentage ± SEM in tumor ascites mononuclear cells. (d and e) Tumor tissues were stained with anti–human B7-H4, anti–human CD3, anti–human-Ham56, and control antibody as described in Materials and methods, and analyzed with confocal microscope. (d) Tumor mass macrophages and tumor cells express B7-H4. B7-H4, green; Ham56, red. Tumor macrophages are identified as Ham56⁺ cells (red). Ham56⁺B7-H4⁺ cells are yellowish. Large numbers of tumor macrophages form a barrier surrounding tumor islets. (e) Tumor-infiltrating T cells are B7-H4⁻. Tumor cells, but not CD3⁺ T cells (red), expressed B7-H4 (green). 1 of 60 representative patient samples is shown for panels d and e.