Figure 5.

CyP inhibits LPS-induced gene expression. Affymetrix methodology was used for global analysis of gene transcription. DCs from two different donors were left untreated (control), treated with 20 μg/ml CyP for 1 or 3 h, or treated with 0.5 μg/ml ultrapure LPS in the absence or presence of CyP for 3 h. (A) Genes showing a statistically significant change (P < 0.05) in at least one experimental condition versus control (321 genes) are ordered on the x axis according to decreasing “fold change” expression (relative to control) induced by LPS treatment. For each gene, the fold changes subsequent to different treatments are shown on the y axis. (B) Volcano plots of the fold changes between treated (1 h CyP, 3 h CyP, and 3 h LPS in the presence or absence of CyP) and untreated DCs for the 12,656 expressed genes. Green, transcripts with at least twofold up-regulation with P < 0.05; blue, transcripts with at least twofold down-regulation with P < 0.05; red, transcripts with fold change less than 2 or P > 0.05. See Table S1 for the list of all expressed gene transcripts.