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. 2006 Jul 10;203(7):1685–1691. doi: 10.1084/jem.20060285

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Copyright © 2006, The Rockefeller University Press

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Figure 1. — IL-1RI is required for induction or activation of antigen-specific ThIL-17 T cells. (A) C57BL/6 or IL-1RI^−/− mice were immunized s.c. with 10 μg KLH either alone or with 10 μg LPS. Inguinal lymph nodes were harvested after 7 d and cells were restimulated with medium or 2, 10, or 50 μg/ml KLH. (B) Lymph node cells were restimulated with medium or 20 ng/ml PMA and 1 μg/ml anti-CD3. Supernatants were collected after 3 d, and IL-4, IL-10, IL-17, and IFN-γ concentrations were determined by ELISA. C57BL/6 versus IL-1RI^−/−: *, P < 0.05; **, P < 0.01; ***, P < 0.001.