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. 2006 Jul 10;203(7):1701–1711. doi: 10.1084/jem.20060772

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Copyright © 2006, The Rockefeller University Press

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Figure 2. — Expression of FoxP3 on different CD4⁺CD127^+/− human T cell subsets. (a) PBMCs were harvested from human peripheral blood and stained with CD4, CD25, CD127 as well as intracellularly with FoxP3-specific mAbs, then analyzed on a Becton-Dickinson FACSCalibur. (b) Human PBMCs were stained for cell surface expression of CD4 and CD127. The stained cells were fixed and stained intracellularly for FoxP3. For analysis, the PBMCs were gated on lymphocytes (based on forward and side light scatter) and analyzed for CD127 and FoxP3 expression. The numbers in the dot plot indicate the percentage of gated cells expressing the relevant marker. Data are representative of >20 independent individuals and >10 experiments.