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. 2006 Jul 10;203(7):1637–1642. doi: 10.1084/jem.20052203

Figure 2.

Figure 2.

Necrotic and apoptotic cells induce HMGB1, TNF, and MIP-2 release in RAW 264.7 cells; Z-VAD-FMK inhibits apoptotic cell-induced HMGB1 release. (A) Normal, apoptotic, or necrotic cells were added to RAW 264.7 cells at the amounts indicated for 16 h at 37°C. HMGB1, TNF, and MIP-2 released in conditioned media were measured. *, P < 0.05 versus normal control group; n = 6–8 independent experiments. (B) Apoptotic cells were added to RAW 264.7 cells at the amounts indicated in the presence of 10 μM Z-VAD-FMK or control peptide for 16 h at 37°C, and the HMGB1 released was measured by Western blotting (9, 10). *, P < 0.05 versus apoptotic cells alone; n = 6–8 independent experiments. Error bars represent SEM.