Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Aug 7;203(8):1985–1998. doi: 10.1084/jem.20060701

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — Anti-GPI B cells in the spleen exhibit an activated MZ phenotype, whereas those in the LN are naive mature follicular cells. Spleen or LN cells from VH147 tg or tg neg B6.K mice were stained with anti-B220, GPI-his, and antibodies against phenotypic markers or activation markers and analyzed by flow cytometry. (A) The percentages of GPI-binding B cells out of total B220⁺ cells in the spleen are given for the boxed regions. The GPI⁺ gate was drawn based on the HELμMT, which contains a monoclonal population of non-GPI-binding B cells. (B) Histograms show surface marker expression for splenic or LN B220⁺GPI⁺ cells from VH147 tg mice (open histograms) overlaid tg neg B cells (shaded histograms). (C) Histograms show activation marker expression for B220⁺GPI⁺ MZ (CD21/35^high), FO (CD21/35^int), or LN VH147 tg (open histograms) overlaid unfractionated tg neg B cells (shaded histograms). (D) Histograms are gated on B220⁺CD21/35^high MZ B cells from the VH147 tg GPI⁺ (open histograms) overlaid GPI⁻ (shaded histograms) populations. n = 5 mice of each genotype.