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. 2006 Aug 7;203(8):1891–1901. doi: 10.1084/jem.20051725

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Copyright © 2006, The Rockefeller University Press

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Figure 4. — IFNα induces expression of Twist. (A and B) Twist1 and Twist2 mRNA levels were measured in control and IFNα-primed macrophages using real-time PCR and were normalized relative to GAPDH levels. (C) Twist2 expression was measured using immunoblotting. (D) Nuclear extracts from control and IFNα-stimulated cells were used in an EMSA assay with a radiolabeled E box containing oligonucleotide. Control and Twist antibodies (2 μg) were added to the binding reaction 20 min before adding radiolabeled oligonucleotide.