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. 2006 Aug 7;203(8):1915–1925. doi: 10.1084/jem.20052085

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Copyright © 2006, The Rockefeller University Press

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Figure 2. — Expression of FcγIIA receptor, phagocytosis, and NADPH oxidase activity. (A) Analysis of FcγIIA expression by flow cytometry using an FITC-conjugated antibody against CD32 is shown for COS7 and COS^phoxFcγR cells (left) and human peripheral blood monocytes (right) as indicated. The gray histogram indicates staining of either COS^phoxFcγR or monocytes with an FITC-conjugated isotype control mAb. (B and C) Phagocytosis of IgG–sheep RBCs in media containing NBT. (B) COS^phoxFcγR cells incubated with IgG-RBCs for 30 min at 37°C. Many of the ingested IgG-RBCs, which appear tan, are indicated by arrows. (C) Murine bone marrow–derived macrophages incubated with IgG-RBCs for 10 min at 37°C. Formazan-stained phagosomes, indicative of intraphagosomal superoxide production, are indicated by arrows. (D) COS^phoxFcγR cells incubated with 100 ng/ml phorbol myristate acetate for 30 min, showing diffuse formazan deposits. Bar, 30 μm.