Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Aug 7;203(8):1915–1925. doi: 10.1084/jem.20052085

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — Expression of p40^phox in COS^phoxFcγR cells and FcγR-elicited NADPH oxidase activity. Data shown is representative of at least three independent experiments. (A) Immunoblots of human neutrophil and COS7 cell lysates (10 μg protein per lane) probed with antibodies for p40^phox, p47^phox, and p67^phox. COS^phoxFcγR cells were transfected with either a stable p40^phox transgene or with varying amounts of p40pRK5 for transient expression as indicated. (B) IgG-RBC–elicited NADPH oxidase activity in COS^phoxFcγR cells expressing p40^phox. Multiple formazan-stained phagosomes (arrows) in COS^phoxFcγR transfected with 0.05 μg p40pRK5 (representative photomicrograph; bar, 30 μm). Similar numbers of formazan-stained phagosomes were present in COS^phoxFcγR cells transfected with larger amounts of plasmid or expressing p40^phox from a stable transgene. (C) Flow cytometry analysis of COSFcγR cell lines incubated with Fc OxyBURST Green–opsonized zymosan for 30 min. Fluorescence intensity is shown on the x axis.