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. 2006 Sep 4;203(9):2201–2213. doi: 10.1084/jem.20052144

Figure 2.

Figure 2.

The novel SDF-1/I-TAC binding protein is RDC1. (A)Binding of 125I SDF-1 or 125I I-TAC to MCF-7 cells was effectively competed using either unlabeled SDF-1 or unlabeled I-TAC. Error bars represent SEM. (B) Binding of 125I SDF-1 to either human breast tumor MDA MB 435s wild-type cells (top) or MDA MB 435s transfected with CXCR7 (bottom) in the presence of increasing concentrations of SDF-1α, I-TAC, CXCR7 antagonist CCX451, and CXCR4 antagonist AMD3100. Total counts bound for each condition are shown and represent the mean of four determinations ± SD. RDC1 Homo sapiens is available from GenBank/EMBL/DDBJ under accession no. P25106. (C) Mouse mAb to human CXCR7 (11G8) binds MDA MB 435s cells transfected with CXCR7 (right) but not MDA MB 435s wild-type cells (left). FACS histogram indicates isotype control (open) and specific staining (shaded). (D) Mouse anti–human CXCR7 mAbs 11G8 and 6E10 but not irrelevant isotype control, 11H3, largely inhibit binding of radiolabeled SDF-1 (top) or I-TAC (bottom) to CXCR7 transfectants. Error bars represent SEM. Data shown in (C) and (D) have been obtained in at least five experiments, and the percent inhibition relative to untreated controls (total bound) is shown.