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. 2006 Sep 4;203(9):2201–2213. doi: 10.1084/jem.20052144

Figure 7.

Figure 7.

CXCR7 mediates cell adhesion in vitro. Adherence is measured (A) by capturing a brightfield image to visualize both the HUVEC monolayer and the labeled adherent cells (bright circles) and (B) by fluorescence to quantitate adhesion. Error bars represent SEM. (C) Activated endothelium expresses CXCR7 by radiolabeled binding assay. HUVECs were stimulated with TNF-α and IL-1β (HUVEC act.) or sham-treated in the presence or absence of cycloheximide (CHX) and incubated with radiolabeled SDF-1. Unlabeled SDF-1α and I-TAC (100 nM each), as well as CCX451 and AMD3100 (10 μM each), were examined for the ability to compete with 125I SDF-1 binding. (D) Northern blot analysis of CXCR7-specific mRNA expressed by unstimulated (HUVEC) or TNF-α/IL-1β (HUVEC act.) cells cultured in the presence or absence of Cycloheximide (CHX). MCF7 (native expression) and CEM-NKr (transfectant) CXCR7 transcripts are shown as controls. Data for A–C were reproduced in at least five experiments. Data for D were obtained twice.