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. 2006 Sep 4;203(9):2063–2071. doi: 10.1084/jem.20061318

Figure 1.

Figure 1.

IGTP-dependent rapid T. gondii elimination in primed macrophages. (A) Impaired T. gondii elimination in primed IGTP−/− mice. CPS-primed (day 7 after infection) WT and IGTP−/− mice were challenged with 4 × 106 GFP-PTG i.p. Infection rates (mean ± SEM) in peritoneal macrophages were determined by FACS (n = 3). (B) Survival of primed WT (n = 6) and IGTP−/− (n = 4) mice after GFP-PTG challenge. (C) Infection rates in primed WT and IGTP−/− peritoneal macrophages after GFP-PTG infection (MOI of 1, 15 min) ex vivo. Data shown are representative of eight experiments. (D) Thioglycolate-elicited WT and IGTP−/− peritoneal macrophages failed to eliminate T. gondii after GFP-PTG infection (MOI of 1, 15 min) when activated in vitro. IFN-γ treatment (1 ng/ml) started 12 h before infection. (E) IGTP-dependent rapid T. gondii elimination in primed macrophages does not require iNOS. Primed (WT, IGTP−/−, iNOS−/−, and IGTP−/−iNOS−/−) and naive WT (thioglycolate-elicited) PECs were infected with GFP-PTG ex vivo. Data are representative of three experiments.