Figure 6.

NF-κB translocation is necessary for host cell survival and bacterial replication. A/J BM macrophages were pretreated for 2 h in the presence or absence of CAPE at 10 μg/ml (reference 39). Cells were incubated with the Dot⁺ or dotA ⁻ strains at MOI = 1, and NF-κB p65 translocation and bacteria were visualized by immunofluorescence microscopy at 1 and 6 hai. Nuclear morphology was observed by Hoechst DNA staining. (A) Examples of p65 staining and nuclear morphology at 6 hai. Bar, 10 μm. (B) NF-κB p65 translocation and cell death (condensed nuclei) were quantitated at 1 or 6 hai. (C) Percentage of condensed nuclei at 15 hai. (D) Bacterial replication at 15 hai. The percentage of cells having phagosomes bearing the denoted number of bacteria is shown. The only cells counted were those harboring bacteria. Means + SE of three coverslips from a representative experiment are given. U, uninfected cells; −, untreated cells; +, cells treated with CAPE.