Figure 7.

PAI-2–dependent and MyD88/Nod1-independent effects associated with L. pneumophila control of NF-κB translocation. (A) BM macrophages derived from C57BL/6 or C57BL/6 pai-2 ^−/− mice were incubated with L. pneumophila Dot⁺-flaA ⁻ at MOI = 1 for the indicated times. Nuclear morphology was observed by Hoechst DNA staining, and the percentage of infected cells with condensed nuclei was quantitated. **, P < 0.0005 as determined by the Student's t test. (B) BM macrophages from C57BL/6 and C57BL/6 myd88 ^−/− mice were incubated with the Dot⁺, Dot⁺-flaA ⁻, or dotA ⁻ strains at MOI = 1 or 5 for the noted times. The percentage of infected cells with NF-κB p65 staining in the nucleus was quantitated by immunofluorescence microscopy. (C) BM macrophages from C57BL/6 nod1 ^+/− heterozygous or C57BL/6 nod1 ^−/− homozygous mice were incubated with the Dot⁺, Dot⁺-flaA ⁻, or dotA ⁻ strains at MOI = 1 for the noted times, and nuclear p65 was determined as in B. Means + SE from three independent experiments are shown.