Table II.
MAP kinase and NF-κB cascade genes are induced by Dot+ infection
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MOI = 10
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MOI = 1
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|---|---|---|---|---|---|---|---|---|---|
| mock | 1 hai dotA − |
8 hai dotA − |
1 hai dotA − |
8 hai dotA − |
1 hai dimB − |
8 hai dimB − |
1 hai Dot+ |
8 hai Dot+ |
|
| MAP kinase pathway | |||||||||
| dusp1 (NM_004417) | 1.9 | 5.2 | 1 | 1.5 | 1.8 | 1.5 | 25.7 | 2.6 | 13.7 |
| dusp2 (NM_0044 18) | 1 | 1.1 | 0.8 | 1.4 | 0.9 | 0.7 | 67.8 | 1.6 | 34.1 |
| dusp6 (NM_001946) | 1.3 | 1 | 1.2 | 1.1 | 1.7 | 0.9 | 3.4 | 1.2 | 3 |
| map2k3 (NM_002756) | 1.4 | 3.3 | 1.3 | 1.1 | 1.4 | 1 | 12 | 1.2 | 3.3 |
| NF-κB pathway genes | |||||||||
| nfkb1 (NM_003998) | 0.8 | 0.7 | 1 | 1.2 | 1.5 | 1.1 | 9.8 | 0.8 | 7.8 |
| nfkb2 (S76638) | 0.7 | 1.1 | 1.3 | 0.8 | 0.8 | 1 | 2.8 | 1 | 2.2 |
| rel (NM_002908) | 0.6 | 0.9 | 0.9 | 0.9 | 1.2 | 0.8 | 2.5 | 0.7 | 2.1 |
| relb (NM_006509) | 1.2 | 0.9 | 1.7 | 1.3 | 2.6 | 1.1 | 8 | 1.4 | 6.9 |
| Activators | |||||||||
| atp2c1 (AF189723) | 1.5 | 0.9 | 0.9 | 1.7 | 0.8 | 0.8 | 4.4 | 1.7 | 2.6 |
| bcl10 (NM_003921) | 1.4 | 1 | 1.6 | 1.2 | 1.6 | 0.7 | 2.1 | 1.8 | 2.3 |
| cl24751 (AF070530) | 0.8 | 0.9 | 1.2 | 1 | 1 | 0.9 | 1.9 | 1.1 | 2.2 |
| ikbkb (AF080158) | 1 | 1.1 | 0.6 | 1.3 | 1.5 | 1.9 | 1.9 | 0.8 | 2.3 |
| irak2 (NM_001570) | 0.5 | 2.8 | 1.7 | 1.3 | 1 | 0.8 | 6.9 | 0.9 | 4.6 |
| malt1 (NM_006785) | 1.5 | 1.1 | 1.1 | 1.3 | 1.5 | 2 | 4.8 | 1.8 | 2.8 |
| oprk1 (NM_000912) | 0.9 | 0.7 | 0.1 | 1.1 | 0.7 | 1.4 | 3.1 | 2.7 | 1.7 |
| tab2 (AL117407) | 1.3 | 2 | 0.9 | 0.9 | 0.8 | 1.1 | 4.9 | 1.6 | 2.3 |
| Inhibitors | |||||||||
| nfkbia (NM_020529) | 1.6 | 9.2 | 3.4 | 3.8 | 5.2 | 3.1 | 27.3 | 2.9 | 36 |
| nfkbie (NM_004556) | 1 | 1.6 | 1.8 | 1.1 | 1.6 | 1.3 | 7.1 | 1.2 | 3.7 |
|
tnfaip3 (A20)
(NM_006290) |
0.8 | 2.7 | 2.7 | 2.3 | 4.3 | 2 | 40.4 | 2.1 | 23.2 |
| Antiapoptotic | |||||||||
| bcl2a1 (NM_004049) | 1 | 1.2 | 1.3 | 1.5 | 1.5 | 1.6 | 2.7 | 1.2 | 2.6 |
| ciap1 (NM_001166) | 1.5 | 0.7 | 0.9 | 1.2 | 2.2 | 0.9 | 1.8 | 1.6 | 3.7 |
| ciap2 (AF070674) | 1 | 0.5 | 2.2 | 0.9 | 1.8 | 5.9 | 32.8 | 1.4 | 14.2 |
| ier3 (NM_052815) | 1 | 4.8 | 1.7 | 1.1 | 0.8 | 1.3 | 6.2 | 1.3 | 3.8 |
| pai-2 (NM_002575) | 1.5 | 4.4 | 2.2 | 0.8 | 2.7 | 0.8 | 7.4 | 0.7 | 7.8 |
Genes in bold were considered to be induced or repressed if the expression was >2-fold higher or <0.5-fold lower in infected macrophage samples compared with the reference (P < 0.05, as determined by the Student's t test). GenBank/EMBL/DDBJ accession numbers are shown in parentheses.