Table III.
Frequency and degree of reconstitution in secondary transplants
| Cell type | Host strain |
Total MNC (1 ×106) |
CD45+
(1 ×106) |
KLS (2 × 103) |
KTLS (2 × 102) |
|---|---|---|---|---|---|
| MAPCs | NOD-SCID | a3/3 (100%) | 5/5 (100%) | ND | a3/4 (75%) |
| (13.3–73.7)b | (0.4–10)b | (0.8–16.4)b | |||
| C57BL | a3/3 (100%) | a3/4 (75%) | a2/3 (67%) | a4/4 (100%) | |
| (15.1–90.7)b | (3.8–94.8)b | (83.0–89.5)b | (17.5–30.1)b | ||
| KTLS | NOD-SCID | c2/2 (100%) | ND | ND | ND |
| (1.7–7.5%)b | |||||
| C57BL | 5/5 (100%) | ND | ND | ND | |
| (9–77.3%)b |
Secondary transplants were performed using cells from GFP+ MAPC- or GFP+ KTLS-grafted primary mice 16–21 wk after transplantation. Total mononuclear cells (MNC) from the BM of primary NOD-SCID or C57BL recipients, and cells enriched for CD45+, GFP+Lin−Sca-1+c-kit+ (KLS), or GFP+Lin−Sca-1+c-kit+Thy-1lo (KTLS) cells were transplanted intravenously into lethally irradiated NOD-SCID or wild-type C57BL-CD45.1+ mice (five recipient animals for each strain). KTLS populations in secondary transfers were co-transplanted with 106 Sca-1–depleted BM cells from mice of host background. Animals were killed 15–24 wk after transplantation, and engraftment levels were determined in the BM as the presence of GFP+CD45.1−CD45.2+ donor-derived cells. The percentage of mice engrafted and chimerism ranges are shown. ND, not done.
Wherever the numerator was <5, mice had died due to radiation sickness.
Range.
Blood chimerism at week 8. Mice died at week 10 due to illness.