Figure 1.

Characterization of CX₃CR1^gfp BM and IBC transfer of MDPs. (A) Flow cytometry analysis of CX₃CR1^gfp/+ BM. The cells in the dot plots to the right are gated according to CD115 and GFP expression. Note the presence of three main CD115⁺ GFP⁺ populations: CD11b⁻ CD117⁺ MDP, CD11b⁺ Gr1^high, and CD11b⁺ Gr1^low BM monocytes. (B) Flow cytometry analysis of an MDP graft isolated by high speed sorting according to CD117 and GFP expression. (C) Analysis of WT recipient BM (right, injected femur) at the indicated time points after IBC transfer of 2.5 × 10⁴ MDPs (purity: 85%, devoid of Gr1^high CD11b⁺ cells). Note the distinct GFP intensity pattern of differentiated graft-derived (CD45.1⁺) CD11b⁺ Gr1^high and Gr1^low BM monocytes and the decrease in the Gr1^high/Gr1^low ratio with time: 14 (day 3) to 2.7 (day 6). (D) Analysis of WT recipient BM (left, noninjected femur) at the indicated time points after IBC transfer. Gating as in C. (E) Analysis of recipient blood (day 6). The cells were gated according to CD115 surface marker expression. The data are representative of at least two independent experiments per time point.