Table II.
Lymphocyte apoptosis using YO-PRO-1 nuclear stain
| No LPS | LPS | LPS/APC | ||
|---|---|---|---|---|
| Spleen tissue | ||||
| T lymphocytes | CD4+ | 0.61 ± 0.37 | 2.20 ± 0.56** | 4.57 ± 3.48 |
| CD8+ | 1.36 ± 0.72 | 4.44 ± 1.01** | 7.11 ± 4.47 | |
| B lymphocytes | B220+ | 1.24 ± 0.07 | 6.42 ± 0.88*** | 7.43 ± 5.17 |
| Granulocytes | GR1+ | 1.03 ± 0.21 | 5.05 ± 2.37* | 7.61 ± 3.93 |
| Peripheral blood | ||||
| T lymphocytes | CD4+ | 0.23 ± 0.06 | 1.69 ± 0.66* | 2.67 ± 0.68 |
| CD8+ | 1.32 ± 0.28 | 7.04 ± 5.73* | 14.4 ± 10.2 | |
| B lymphocytes | B220+ | 0.66 ± 0.14 | 4.45 ± 2.23* | 5.04 ± 2.71 |
| Granulocytes | GR1+ | 0.07 ± 0.06 | 0.09 ± 0.34 | 1.28 ± 1.07 |
All collections were performed 24 h after LPS challenge and APC treatment . *, P ≤ 0.05; **, P ≤ 0.01; and ***, P ≤ 0.001 compared with PBS control mice using the Student's t test.