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. 2007 Oct 1;204(10):2293–2303. doi: 10.1084/jem.20070583

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Copyright © 2007, The Rockefeller University Press

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Figure 5. — The frequency of d- or J-side RSS insertion into the b196 hotspot in wild-type and mutant mice. 85 1-μg (∼166,000 genome) samples of thymus or spleen DNA from the indicated mice were analyzed by PCR for either D or J RSS hotspot insertion events. Frequency was calculated according to the Poisson distribution. Error bars on the cR2 Thy and WT Thy samples indicate analysis of independent sets of 85 PCR assays from each of 3 different animals.