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. 2007 Oct 1;204(10):2349–2362. doi: 10.1084/jem.20062596

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Copyright © 2007, The Rockefeller University Press

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Figure 6. — Sites of leukocyte entry into initial lymphatics in airway inflammation. (A) Whole mount of mouse trachea 24 h after intratracheal LPS. MHC II–positive cell clusters (arrows, red) in or near initial lymphatics (green). (B) Enlargement of boxed region in A. Cells inside lymphatic (arrows) are more rounded than dendritic cells in trachea of pathogen-free mouse (inset). (C) Distribution of MHC II–positive cell clusters along the length of tracheal lymphatics, with the tip used as a reference. Half of the cell clusters were within 160 μm of the tip. Values are presented as means ± SEM. (D and E) Isosurface renderings of confocal images of MHC II cells (arrows) entering an initial lymphatic with buttons. (E) Enlargement of boxed region in (D). (F) MHC II–positive cells near and inside initial lymphatic of a PECAM-1–null mouse 24 h after LPS. (G) Transmission electron microscopic image of a leukocyte (pink) migrating through an intercellular junction in endothelium (green) of tracheal lymphatic with prominent junctional flap (arrow; M. pulmonis infection, 6 wk). (H and I) Confocal image (H) and isosurface rendering (I) of CD45-positive leukocytes (red, arrows) inside initial lymphatic 24 h after infection by M. pulmonis. Endothelial cell junctions are marked by VE-cadherin (red). PECAM-1, green; LYVE-1, blue. See also Video 1, available at http://www.jem.org/cgi/content/full/jem.20062596/DC1. Bars: (A) 200 μm; (B) 50 μm; (D) 10 μm; (E) 5 μm; (F) 50 μm; (G) 2 μm; (H and I) 20 μm.