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. 2007 Oct 29;204(11):2615–2627. doi: 10.1084/jem.20070318

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Copyright © 2007, The Rockefeller University Press

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Figure 8. — Bcl-3 accumulation in CYLD^ex7/8 B cells. (A) Cytospins were prepared from B cells isolated from CYLD^ex7/8, CYLD^ex7/8;wt, CYLD^ko, and WT mice and immunostained with anti–Bcl-3 and counterstained with Hoechst 33258. Shown are unstimulated B cells (top) and B cells treated with anti-BCR (bottom). Bar, 5 μm. (B) Same as in A, but showing more cells. Bar, 5 μm. (C) Quantification of nuclear Bcl-3 from unstimulated B cell cytospins from the indicated genotypes. For each individual genotype, 400 cells were counted and quantified for nuclear Bcl-3 localization. The frequency of nuclear Bcl-3 localization in CYLD^ex7/8 B cells were statistically significant (P < 0.001) compared with B cells isolated from WT, CYLD^ex7/8;wt, and CYLD^ko mice. (D) Lysates of MACS-purified B cells from the indicated genotypes subjected to Western blot analysis using Bcl-3 antibody. Duplicates for each mouse strain are shown. Actin was used as a loading control.