Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Oct 29;204(11):2629–2639. doi: 10.1084/jem.20071262

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 6. — Degradation of S6b by mast cells. (A–D) Mass spectrometric analyses (left side) of degradation products of S6b. Resulting peptides are schematically depicted on the right side. S6b was left untreated (A) or incubated with ionomycin-stimulated purified mast cells from Mc-cpa^+/+ (B) or Mc-cpa^Y356L,E378A (C and D) mice. C-terminal degradation, which is evident from the appearance of fragments corresponding to the molecular mass of S6b (1–19; 2,266 daltons), was mediated by Mc-cpa^+/+ (B), but not by Mc-cpa^Y356L,E378A (C) mast cells. The peak marked by the asterisk in B does not correspond to a fragment of S6b, and it was not observed in a second experiment. The molecular mass of S6b (1–21) increased after incubation with Mc-cpa^Y356L,E378A mast cells from 2,565 (1–21; A) to 2,583 daltons (S6b 1–21 plus water; C). This indicated hydrolysis of S6b, which was proven after reduction of the samples by the disappearance of the S6b 1–21 peak, and the appearance of residual fragments with molecular masses of 1,710 and 1,858 daltons (see Materials and methods) corresponding to S6b 1–13 and 1–14, respectively (D). Data are representative for two independent experiments.