Table I.
Binding of threshold ligands to thymocytes from three different TCR transgenic mouse strains (T1, S14, and OT-1 TCR)
TCR /ligand | pMHC monomers
|
pMHC tetramers
|
|
---|---|---|---|
DP thymocytes (K d [M]) |
SP thymocytes (K d [M]) |
SP thymocytes (r 1/2 [M]) |
|
T1/4A-Kd | 5.4 ± 1.6 × 10−6 | 5.5 ± 1.8 × 10−6 | Not done |
T1/4S-Kd | 6.1 ± 1.1 × 10−6 | 7.9 ± 2.0 × 10−6 | 9.0 ± 1.5 × 10−8 |
S14/4L-Kd | 6.4 ± 1.5 × 10−6 | 6.2 ± 1.7 × 10−6 | 8.8 ± 1.6 × 10−8 |
OT-1/OvaT4-Kb | Not done | Not done | 7.6 ± 0.7 × 10−8 |
Mean values and SEMs of at least three independent experiments are shown.