Table II.
K d values (M) determined for pMHC monomers bound to T1 thymocytes and lymph node T cells
| Cell type | Ligands (pMHC monomers)
|
||||||
|---|---|---|---|---|---|---|---|
| 4L-Kd
K d (M) |
4P-Kd
K d (M) |
4V-Kd
K d (M) |
4A-Kd
K d (M) |
4S-Kd
K d (M) |
4N-Kd
K d (M) |
4H-Kd
K d (M) |
|
| 0°C | |||||||
| Preselection DP thymocyte |
6.8 × 10−10 | 2.9 × 10−9 | 3.2 × 10−8 | 2.2 × 10−7 | 2.2 × 10−7 | 7.1 × 10−7 | 3.5 × 10−6 |
| Postselection DP thymocyte |
1.8 × 10−9 | 6.1 × 10−9 | 5.9 × 10−8 | 5.0 × 10−7 | 5.2 × 10−7 | 2.2 × 10−6 | 5.9 × 10−6 |
| SP thymocyte | 1.4 × 10−9 | 5.3 × 10−9 | 3.3 × 10−8 | 5.6 × 10−7 | 3.7 × 10−7 | 2.3 × 10−6 | 7.8 × 10−6 |
| LN T cell | 1.5 × 10−9 | 6.8 × 10−9 | 3.0 × 10−8 | 4.5 × 10−7 | 3.3 × 10−7 | 1.6 × 10−6 | 6.0 × 10−6 |
| Δ Kd (SP/DP) | 0.8 | 0.9 | 0.6 | 1.1 | 0.7 | 1.0 | 1.3 |
| Δ Kd (LN/DP) | 0.8 | 1.1 | 0.5 | 0.9 | 0.6 | 0.7 | 1.0 |
| 37°C | |||||||
| Preselection DP thymocyte |
2.8 × 10−8 | 8.8 × 10−8 | 2.6 × 10−7 | 2.2 × 10−6 | 2.9 × 10−6 | 2.9 × 10−5 a | >6 × 10−5 a |
| Postselection DP thymocyte |
6.1 × 10−8 | 1.7 × 10−7 | 4.3 × 10−7 | 5.4 × 10−6 | 6.1 × 10−6 | 5.5 × 10−5 a | >6 × 10−5 a |
| SP thymocyte | 4.0 × 10−8 | 1.1 × 10−7 | 3.5 × 10−7 | 5.5 × 10−6 | 7.9 × 10−6 | 5.8 × 10−5 a | >6 × 10−5 a |
| LN T cell | 2.5 × 10−9 | 1.2 × 10−7 | 3.6 × 10−7 | 4.7 × 10−6 | 5.8 × 10−6 | 3.1 × 10−5 a | >6 × 10−5 a |
| Δ Kd (SP/DP) | 0.7 | 0.6 | 0.8 | 1.0 | 1.3 | nd | nd |
| Δ K d (LN/DP) | 0.4 | 0.7 | 0.8 | 0.9 | 0.9 | nd | nd |
Mean values of at least three independent experiments are shown. nd, not determined. DK d represents the fold difference in ligand affinities between T lineage cells at the indicated developmental stages.
a
Extrapolated values.