Skip to main content
. 1998 Jul 21;95(15):8963–8968. doi: 10.1073/pnas.95.15.8963

Figure 3.

Figure 3

Tissue distribution of NaN in adult rat by RT-PCR (A) and Northern blot analysis (B). (A) “M” lanes contain the 100-bp ladder marker (Pharmacia). Amplification product from DRG (lanes 1 and 16), trigeminal ganglia (lane 9), cerebral hemispheres (lane 2), and retina (lane 4) are consistent with a predicted size of 392 bp. No detectable signal is seen in cerebellum (lane 3), optic nerve (lane 5), spinal cord (lane 6), sciatic nerve (lane 7), superior cervical ganglia (lane 8), skeletal muscle (lane 10), cardiac muscle (lane 11), adrenal gland (lane 12), uterus (lane 13), liver (lane 14), kidney (lane 15), or water (lane 17). (B Upper) An antisense riboprobe hybridized specifically to a single transcript (about 6.5 kb) in poly(A)+ DRG RNA. No similar hybridization signal was seen in multiple-tissue Northern blot (CLONTECH) lanes containing poly(A)+ RNA from heart, brain, spleen, lung, liver, muscle, kidney, and testis. (B Lower) β-actin (1.6 kb and 2.0 kb) hybridization signal. Heart and muscle lanes show an actin signal at 1.6 kb, consistent with hybridization to the α- or γ-actin forms in these tissues (Boehringer Mannheim).