Figure 4.

NaN mRNA expression in adult rat tissue by in situ hybridization. Strong hybridization signal for NaN mRNA is present in many small-diameter neurons within trigeminal ganglia (a) and DRG (b). Large-diameter neurons (arrow) generally lack NaN mRNA hybridization signal. DRG neurons hybridized with NaN sense riboprobe do not show signal above background (c). Hybridization signal for NaN mRNA is not present in cerebellum (d) or in liver, spinal cord, heart, kidney, and adrenal (not shown). (Bar = 50 μm.)