Figure 4.

Inhibition of the maturation of MDDCs by HLA-B2705–KK10 L6M complexes. (A and B) Histograms indicating the expression intensity of CD40, CD86, and HLA-DR of myelomonocytic cells after cytokine-induced maturation in the presence or absence of HLA-B2705–KK10 WT or B2705–KK10 L6M pentamers (A) or autologous HLA-B2705–expressing DCs pulsed with the KK10 WT or KK10 L6M variant peptide (B). One example is shown. (C) Mean and standard deviation of the fluorescence intensity of DC maturation markers and co-stimulatory molecules after cytokine cocktail–mediated maturation in the presence or absence of the B2705–KK10 WT or L6M pentamers. Experiments were independently performed four times. (D) Assessment of HLA-B27–KK10 L6M–mediated functional inhibition of DCs by mixed lymphocyte reactions. Dot plots indicate the proportion of allogenic CD4 or CD8 T cells after a 6-d exposure to MDDCs in the presence of HLA-B27–KK10 WT or L6M pentamers. One experiment out of three in three different study subjects is shown.