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. 2007 Nov 26;204(12):3037–3047. doi: 10.1084/jem.20070885

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Copyright © 2007, The Rockefeller University Press

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Figure 3. — Sequential recruitment of Ly-6C^hi and -6C^lo monocytes depends on CCR2 and CX₃CR1, respectively. (A) RT-PCR expression profile of MCP-1, MIP-1α, fractalkine, and VCAM-1 in the heart tissue before MI and during phase I (day 1) and II (day 4). (B) Monocyte subset expression profile of CCR2, CCR5, CX₃CR1 (for review see reference [29]), and VLA-4 (as assessed by flow cytometry). (C) Number of Ly-6C^hi and -6C^lo monocytes in infarcts of wild-type, CCR2^−/−, and CX₃CR1^−/− mice in phase I (day 1) and II (day 7). Numbers are normalized to milligrams of tissue. The mean and the SEM are shown. n = 3–5. *, P < 0.05.