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. 2007 Nov 26;204(12):3037–3047. doi: 10.1084/jem.20070885

Figure 5.

Figure 5.

In vivo relevance of the biphasic response to healing. (A) Representative dot plots from individual mice depict Ly-6Chi monocytes (bottom right), Ly-6Clo monocytes (bottom left), and macrophages/dendritic cells (top left) at the infarct after depletion of circulating monocytes with clodronate-loaded liposomes (Clo-Lip). Mice were analyzed on day 4 (Clo-Lip injection on day 0; depletion of phase I) and on day 7 (Clo-Lip injection on day 3; depletion of phase II). Control animals (Ø) did not receive Clo-Lip. Percentages of cells are shown as the mean ± the SEM. (B) Total number of monocytes per milligram of tissue at the infarct before MI, at the end of phase I (day 4) and during phase II (day 7), in the absence (–) or presence (+) of Clo-Lip. The mean and the SEM are shown. n = 3–5. (C) Immunohistochemical analysis 7 d after MI depicts representative infarct sections from undepleted (Ø), phase I–depleted (I), and phase II–depleted (II) C57BL/6 mice. Representative sections stained with anti–Mac-3, anti–NIMP-R14, Masson, α-actin, PSR, and anti-CD31 are shown. The mean and the SEM are shown. n = 7. (D) Immunohistochemistry depicts representative infarct sections from apoE−/− mice 7 d after MI. The mean and the SEM are shown. n = 5. *, P < 0.05; **, P < 0.01. Bars: (Mac-3, NIMP-R14, α-actin, and CD31) 20 μm; (PSR and Masson) 100 μm.