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. 2007 Nov 26;204(12):2875–2888. doi: 10.1084/jem.20062299

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Copyright © 2007, The Rockefeller University Press

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Figure 2. — Failure of cell-cycle arrest in DP thymocytes of Lck-Cre/Fbxw7^F/Fmice. (A) Gross appearance of the thymus of Lck-Cre/Fbxw7^+/F and Lck-Cre/Fbxw7^F/F mice at 8 wk of age. Bar, 5 mm. (B) Representative flow cytometric analysis of surface expression of CD4 and CD8 on thymocytes from Lck-Cre/Fbxw7^+/F or Lck-Cre/Fbxw7^F/F mice at 8 wk of age. The percentages of DN, DP, and SP populations are indicated. (C) Absolute cell numbers for total thymocytes and thymocyte subsets determined as in B. Data are means ± SD of values from 13 Fbxw7^F/F (control) and 19 Lck-Cre/Fbxw7^F/F mice. **, P < 0.01 using the Student's t test. (D) BrdU incorporation into thymocytes in vivo. The proportion of each thymocyte subset in S phase was determined by measurement of incorporation of BrdU after its intraperitoneal injection in 8-wk-old Lck-Cre/Fbxw7^F/F or Fbxw7^F/F (control) mice. Data are means ± SD of values from four animals of each genotype. **, P < 0.01 using the Student's t test. (E) Increased expression of Notch1, Notch3, and c-Myc in total thymocytes from Lck-Cre/Fbxw7^F/F mice. Total thymocytes of 8-wk-old Lck-Cre/Fbxw7^+/F or Lck-Cre/Fbxw7^F/F mice were subjected to immunoblot analysis with antibodies to the indicated proteins. (F) Accumulation of Notch1, Notch3, and c-Myc specifically in DP thymocytes of Lck-Cre/Fbxw7^F/F mice. Thymocyte subsets of Fbxw7^F/F (control) or Lck-Cre/Fbxw7^F/F mice were analyzed as in E.