Figure 2.

Expression kinetics of steady-state mRNA encoding transcription factors during memory B cell and plasma cell differentiation in vitro. Cells from plasma and memory B cell cultures were harvested on the days indicated. Total cells were harvested and RNA was prepared. Quantitative RT-PCR for BCL-6, Pax5, Bach2, BLIMP-1, XBP-1, and IRF-4 was performed. All values were normalized to cyclophilin A and are shown relative to the value at day 0. Each point is the mean of triplicates in qRT-PCR for each experiment. These profiles are representative of three separate experiments.