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. 2007 Apr 16;204(4):819–830. doi: 10.1084/jem.20062104

Figure 3.

Figure 3.

Expression of Bcl-6 mRNA and protein is low in centrocyte and memory cultures. (A) Purified centroblasts at Day 0 (centroblasts [CB]) or cultured for 3 d with CD40L, IL-2 (centrocytes [CC]), or CD40L, IL-2, IL-4 (memory B cells [MEM]) were harvested and qRT-PCR was performed for BCL-6 and cyclophilin A. Representative of at least five independent experiments. Error bars correspond to the standard deviation. (B) Whole cell extracts of 100,000 cells from each culture described (see Materials and methods) were used for immunoblotting with antibodies to BCL-6 and actin. Representative of two independent experiments.