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. 2007 May 14;204(5):1145–1156. doi: 10.1084/jem.20061952

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Copyright © 2007, The Rockefeller University Press

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Figure 3. — AID expression in AID-GFP and AID-GFP-ΔX transgenic mice. (A) Molecular schemes of AID-GFP and AID-GFP-ΔX transgenes. The position of CNS X is indicated. (B) FACS analysis of AID expression in wild-type (left) and transgenic mice (middle and right) immunized with NP-CGG and CFA. Cells were stained with CD95-PE and gated on B220 (PercP-Cy5.5). Apoptotic cells were excluded by To-Pro-3 or DAPI staining. The GC nature of GFP⁺ cells was confirmed by GL7-APC staining (not depicted). (C) Endogenous AID (gray bars), AID-GFP (green bars), and AID-GFP-ΔX (red bars) transcripts were measured by quantitative PCR from resting, LPS, or LPS plus IL-4 stimulated B lymphocytes. The diagram on the left depicts primer positions.